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Proteome variation of the rat liver after static cold storage assayed in an ex vivo model |
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| Institution: | 1. Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, 2000, Argentina;2. Centro Binacional (Argentina-Italia) de Investigaciones en Criobiología Clínica y Aplicada (CAIC), Universidad Nacional de Rosario, Rosario, 2000, Argentina;1. Surgical Research Laboratory, Department of Surgery, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands;2. Section of Hepato-Pancreato-Biliary Surgery and Liver Transplantation, Department of Surgery, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands;3. Section of Hepato-Pancreato-Biliary Surgery and Liver Transplantation, Department of Surgery, University of Massachusetts Medical School, University of Massachusetts Medical Center, University of Massachusetts, Worcester, MA, USA;1. Institute for Laboratory Animal Science and Experimental Surgery, RWTH-Aachen University, Aachen, Germany;2. Department of Hepatobiliary, Pancreas and Transplant Surgery, Kyoto University, Kyoto, Japan;3. Division of Pathology, Institute for Laboratory Animal Science and Experimental Surgery, RWTH-Aachen University, Aachen, Germany;1. Experimental Hepatic Ischemia-Reperfusion Unit, Institut d’Investigacions Biomèdiques de Barcelona (IIBB), CSIC-IDIBAPS, Barcelona, Spain;2. Research Unit of Biology and Molecular Anthropology Applied to Development and Health (UR12ES11), Faculty of Pharmacy, University of Monastir, Tunisia;3. High Institut of Biotechnology of Monastir, University of Monastir, Tunisia;4. Centre Hépato-Biliaire, Hôpital Universitaire Paul Brousse, Villejuif, France;1. Department of Hepatobiliary Surgery, The Affiliated Hospital of Luzhou Medical College, Jiangyang, Luzhou, Sichuan, China;2. Department of Anesthesia, The Affiliated Hospital of Luzhou Medical College, Jiangyang, Luzhou, Sichuan, China;1. Hepatobiliary Surgery and Liver Transplantation Unit, University of Pisa Medical School Hospital, Pisa, Italy;2. Liver Transplant Intensive Care Unit, University of Pisa Medical School Hospital, Pisa, Italy;3. Division of Pathology, University of Pisa Medical School Hospital, Pisa, Italy;4. Division of Immunohematology, University of Pisa Medical School Hospital, Pisa, Italy;5. Translational Research Department, University of Pisa Medical School Hospital, Pisa, Italy;6. Division of Endocrinology and Metabolism in Organ Transplantation, University of Pisa Medical School Hospital, Pisa, Italy |
| Abstract: | Cold storage is a common procedure for liver preservation in a transplant setting. However, during cold ischemia, the liver suffers molecular alterations that can affect its performance. Also, deleterious mechanisms set forth in the storage phase are exacerbated during reperfusion. This study aimed to identify liver proteins associated with injury during cold storage and/or normothermic reperfusion using the isolated perfused rat liver model. Livers from male rats were subjected to either (1) cold storage for 24 h, (2) ex vivo normothermic reperfusion for 90 min or (3) cold storage for 24 h followed by ex vivo normothermic reperfusion for 90 min. Then, the livers were homogenized and proteins were extracted. Protein expression between each experimental group and the control (freshly resected livers) was compared by two-dimensional (2D) gel electrophoresis. Protein identification was carried out by matrix‐assisted laser desorption/ionization time‐of‐flight spectrometry (MALDI‐TOF/TOF) using MASCOT as the search engine. 23 proteins were detected with significantly altered levels of expression among the different treatments, including molecular chaperones, antioxidant enzymes, and proteins involved in energy metabolism. Some of them have been postulated as biomarkers for liver damage while others had been identified in other organs subjected to ischemia and reperfusion injury. The whole data set will be a useful resource for studying deleterious molecular mechanisms that result in diminished liver function during storage and subsequent reperfusion. |
| Keywords: | Two-dimensional gel electrophoresis Reperfusion injury Cold ischemia Proteomics Liver COMT"} {"#name":"keyword" "$":{"id":"kwrd0040"} "$$":[{"#name":"text" "_":"catechol-O-methyltransferase cytokeratin 8"} {"#name":"keyword" "$":{"id":"kwrd0050"} "$$":[{"#name":"text" "_":"K8 EST"} {"#name":"keyword" "$":{"id":"kwrd0060"} "$$":[{"#name":"text" "_":"estrogen sulfotransferase hnRNP K"} {"#name":"keyword" "$":{"id":"kwrd0070"} "$$":[{"#name":"text" "_":"heterogeneous nuclear ribonucleoprotein K HTK"} {"#name":"keyword" "$":{"id":"kwrd0080"} "$$":[{"#name":"text" "_":"histidine-tryptophan-ketoglutarate IPRL"} {"#name":"keyword" "$":{"id":"kwrd0090"} "$$":[{"#name":"text" "_":"isolated perfused rat liver IRI"} {"#name":"keyword" "$":{"id":"kwrd0100"} "$$":[{"#name":"text" "_":"ischemia/reperfusion injury L-PK"} {"#name":"keyword" "$":{"id":"kwrd0110"} "$$":[{"#name":"text" "_":"liver-type pyruvate kinase MALDI-TOF"} {"#name":"keyword" "$":{"id":"kwrd0120"} "$$":[{"#name":"text" "_":"matrix-assisted laser desorption/ionization time-of-flight NDRG2"} {"#name":"keyword" "$":{"id":"kwrd0130"} "$$":[{"#name":"text" "_":"N-myc downstream-regulated gene 2 SCS"} {"#name":"keyword" "$":{"id":"kwrd0140"} "$$":[{"#name":"text" "_":"static cold storage SD"} {"#name":"keyword" "$":{"id":"kwrd0150"} "$$":[{"#name":"text" "_":"standard deviation two-dimensional"} {"#name":"keyword" "$":{"id":"kwrd0160"} "$$":[{"#name":"text" "_":"2D |
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