First steps towards Echinometra lucunter embryo cryopreservation |
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| Institution: | 1. Instituto APLYSIA, Rua Júlia Lacourt Penna Nº 335, Jardim Camburi, Vitória, 29.090 – 210, Brazil;2. Instituto Federal do Espírito Santo, Rua Augusto Costa de Oliveira Nº 660, Praia Doce, Piúma, 29285-000, Brazil;3. Marine Biological Resources Functional Preservation Service, Estación de Ciencias Mariñas de Toralla, Universidade de Vigo, Illa de Toralla 36331, Coruxo, Vigo, Spain;1. UR: Physiology and Aquatic Environment, Department of Biology, Faculty of Sciences of Tunis, Univ. Tunis El Manar, 2092 Tunis, Tunisia;2. BVBGR-LR11ES31, Higher Institute of Biotechnology - Sidi Thabet, Biotechpole Sidi Thabet, Univ. Manouba, 2020 Ariana, Tunisia;3. National Institute of Marine Science and Technology (INSTM), La Goulette Center, 2060 Tunis, Tunisia;1. Aix Marseille Univ, Avignon Université, CNRS, IRD, IMBE, Marseille, France;2. Zoological Institute, University of Basel, Vesalgasse 1, 4051 Basel, Switzerland;3. Swedish Museum of Natural History, Department of Zoology, Box 50007, 10405 Stockholm, Sweden |
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| Abstract: | We have studied the sensitivity to cryoprotecting agents of different embryos of the local sea urchin, Echinometra lucunter which is the species used for embryo-larval bioassays in Brazil. We have located significant differences between both species sensitivity to cryoprotecting agents; while for P. lividus propylene glycol was the less toxic compound for most development stages, whereas for E. lucunter is was the most toxic and the least toxic was Dimethyl sulfoxide. There is a significant difference between development stages as well; in the case of P. lividus, the blastula embryo was the most resistant to the cryoprotecting agents, meanwhile for E. lucunter, it was the fertilized oocyte. This is a very promising result, a really early embryo that is not extremely sensitive to Me2SO. Our next aim is to develop a cryopreservation protocol for E. lucunter early embryos and test them in an embryo-larval bioassay. |
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| Keywords: | Sea urchin Cryopreservation Cryoprotecting agents Marine invertebrate Embryos |
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