Effect of sericin supplementation on heat shock protein 70 (HSP70) expression,redox status and post thaw semen quality in goat |
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| Institution: | 1. Department of Veterinary Physiology, College of Veterinary Science and Animal Husbandry, Veterinary University, Mathura, Uttar Pradesh, India;2. Department of Veterinary Gynecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Veterinary University, Mathura, Uttar Pradesh, India;3. College of Biotechnology, Veterinary University, Mathura, Uttar Pradesh, India;1. Transgenesis Center of Excellence, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran;2. Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;3. Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran;1. Animal Physiology Division, ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru, Karnataka 560030, India;2. Animal Nutrition Division, ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru, Karnataka 560030, India;1. Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;2. Department of Clinical Sciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan;3. Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran;1. Department of Veterinary Medicine, University of Sassari, Sassari, Italy;2. Centro di Competenza Biodiversità Animale, Sassari, Italy;1. ICAR-National Research Centre on Mithun, Medziphema, 797106, Nagaland, India;2. ICAR-ICAR Research Complex for NEH Region, Umiam, 793103, Meghalaya, India;3. Germ-Plasm Centre, ICAR-Indian Veterinary Research Institute, Bareilly, 243122, UP, India;1. College of Animal Science and Technology, Northwest A&F University, Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, Yangling, Shaanxi, 712100, PR China;2. Center of Northern Shaanxi White Cashmere Goats Engineering Research, Yulin University, Yulin, Shaanxi 719000, China;3. Center of Lhasa Animal Disease Prevention and Control, Lhasa, Tibet, 850000, China |
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| Abstract: | Cryopreservation results in substantial deterioration of heat shock protein 70 (HSP70) and ultra-structural changes in sperm organelles, resulting in a marked reduction in post-thaw semen quality. The present study was aimed to explicate the effect of sericin supplementation on expression profile of HSP70, redox status and post-thaw semen quality in Barbari goat. Five Barbari bucks were used to collect thirty semen ejaculates by using artificial vagina and each ejaculate was divided into three aliquots to which sericin was supplemented at 0% (Control), 0.25% (T1) and 0.50% (T2). Further, extended semen samples were equilibrated followed by their cryopreservation. Post-thaw semen characteristics, redox status of seminal plasma, enzyme leakage and HSP70 gene/protein expression in spermatozoa were assessed in all the groups. Per cent progressive motile spermatozoa, spermatozoa having intact plasma membrane (HOST + ve) and intact acrosomes in post-thaw spermatozoa were significantly (p < 0.01) higher in T1 and T2 as compared to control. A significant (p < 0.01) reduction in abnormal spermatozoa was found in T1 as compared to T2. Sericin supplementation significantly (p < 0.05) improved the antioxidative status (SOD, GST, CAT), reduced lipid peroxidation (MDA) and also prevented enzyme (ALT, LDH) leakage as compared to control samples. qRT-PCR results revealed that HSP70 mRNA expression was significantly (p < 0.01) upregulated in T1 and T2 group as compared to control. The positive effect of sericin on expression of HSP70 was further confirmed by immunoblotting followed by densitometry revealing higher expression in T1 and T2 compared to control. Inclusion of 0.25% w/v sericin in semen extender ameliorated the post-thaw semen quality by improving antioxidative status and minimizing the leakage of intracellular enzymes. Sericin supplementation had a beneficial effect on HSP70/HSP70 mRNA expression either by induction or by protection of HSP70/HSP70 mRNA as evident from the gene expression and immunoblotting studies. |
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| Keywords: | Goat Spermatozoa Cryopreservation Sericin HSP70 |
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