Immunological response induced by cryoablation against murine H22 hepatoma cell line in vivo |
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| Affiliation: | 1. Department of Interventional Therapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center of Cancer, Tianjin''s Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, China;2. Beijing Chao-Yang Hospital, Beijing, 100020, China;1. Departments of Interventional Oncology Guangzhou, China;2. Interventional Radiology, Guangzhou, China;3. Medical Imaging, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China;4. Fuda Cancer Hospital, Jinan University School of Medicine, Guangzhou, China;5. Fuda Institute of Cryosurgery for Cancer, Guangzhou, China;1. Department of Gastroenterology and Hepatology, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan;2. Department of Gastrointestinal and Pediatric Surgery, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan;3. Department of Anatomic Pathology, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan;4. Department of Gastroenterology and Hepatology, Tokyo Medical University Hachioji Medical Center, Tokyo;5. Department of Intelligent Image Information, Gifu University Graduate School of Medicine, Gifu, Japan;1. Department of Interventional Radiology, UF Health Jacksonville, University of Florida, 655 W 8th, St., Jacksonville, FL, 32209, USA;2. Department of Urology, UF Health Jacksonville, University of Florida, 655 W 8th, St., Jacksonville, FL, 32209, USA;1. Department of Biological Treatment Center, Fuda Cancer Hospital, Jinan University School of Medicine, No 2, Tangde Xi Road, Tianhe District, Guangzhou, China;2. Fuda Cancer Institute, No 2,Tangde Xi Road, Tianhe District, Guangzhou, China;3. Hank Bioengineering Co., Ltd, Shenzhen, No 72, Guowei Road, Luohu District, Shenzhen, China |
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| Abstract: | ObjectivesTo describe immunological consequences induced by cryoablation against H22 cells in vivo.MethodsAdult BALB/c mice underwent subcutaneous implantation of H22 cells. All of them were assigned into three groups randomly: group A (false surgery), group B (cryoablation) and group C (cryoablation plus Freund's adjuvant). Animals were sacrificed 1, 2 and 3 weeks after treatment. Serum IFN-γ and IL-4, Th1/Th2 in spleens and cytotoxicity were detected.ResultsCompared with that of group A, (1) INF-γ of group B was higher, but IL-4 was lower; cryoablation plus Freund's adjuvant enhanced these effects. (2) Th1/Th2 rose significantly in both group B and group C. (3) Strong cytolytic activity against H22 cells of group B and group C was found on day 7, 14 and 21.ConclusionsOur study showed a marked shift toward Th1 and IFN-γ expression after cryoablation, with an immuno-stimulatory effect against murine H22 hepatoma Cell. |
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| Keywords: | Cryoablation Hepatocarcinoma Immunological response Hepatocellular carcinoma" },{" #name" :" keyword" ," $" :{" id" :" kwrd0030" }," $$" :[{" #name" :" text" ," _" :" HCC interferon-y" },{" #name" :" keyword" ," $" :{" id" :" kwrd0040" }," $$" :[{" #name" :" text" ," _" :" INF-γ Interleukin–4" },{" #name" :" keyword" ," $" :{" id" :" kwrd0050" }," $$" :[{" #name" :" text" ," _" :" IL-4 enzyme-linked immunosorbent assay" },{" #name" :" keyword" ," $" :{" id" :" kwrd0060" }," $$" :[{" #name" :" text" ," _" :" ELISA natural killer cell" },{" #name" :" keyword" ," $" :{" id" :" kwrd0070" }," $$" :[{" #name" :" text" ," _" :" NK cytotoxic T lymphocyte" },{" #name" :" keyword" ," $" :{" id" :" kwrd0080" }," $$" :[{" #name" :" text" ," _" :" CTL |
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