Cryoprotective role of organic Zn and Cu supplementation in goats (Capra hircus) diet |
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| Affiliation: | 1. Animal Physiology Division, ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru, Karnataka 560030, India;2. Animal Nutrition Division, ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru, Karnataka 560030, India;1. Université de Lyon, VetAgro Sup, Interaction cellule environnement, Marcy l’Etoile, France;2. Université de Lyon, ISARA-Lyon, Lyon, France;1. University Maternal Hospital, Department of Obstetrics and Gynaecology, Cologne University, Kerpener Str. 34, 50931, Cologne, Germany;2. Cell Sort Service Department, Center for Molecular Medicine Cologne University (CMMC), Robert Koch Str. 21, 50931, Cologne, Germany;1. Transgenesis Center of Excellence, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran;2. Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;3. Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran;1. National Institute for Agricultural and Veterinary Research (I.N.IA.V., I.P.), Santarém, Portugal;2. The University of Sydney, Faculty of Science, School of Life and Environmental Sciences, NSW 2006, Australia;1. Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;2. Department of Clinical Sciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan;3. Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran;1. Departamento de Fisiología, Facultad de Veterinaria, Universidad de la República, Lasplaces 1550, Montevideo, 11600, Uruguay;2. Departamento de Biología Molecular y Celular, Facultad de Veterinaria, Universidad de la República, Lasplaces 1550, Montevideo, 11600, Uruguay;3. Departamento de Reproducción Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Madrid, Spain |
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| Abstract: | The current study focused on cryopreservation and assessment of characters of post-thaw semen of indigenous Osmanabadi bucks maintained with standard diet, supplemented with different concentrations of organic zinc (Zn), copper (Cu) or in combination, for a period of 180 days. The different doses of organic Zn and Cu were fed per kg DM basis, Zn groups (low: Zn20, medium: Zn40 and high: Zn60), Cu groups: (low: Cu12.5, medium: Cu25 and high: Cu37.5) and combination of Zn + Cu groups (low: Zn20 + Cu12.5, medium: Zn40 + Cu25 and high: Zn60 + Cu37.5) respectively. The control group bucks were maintained mainly on the basal diet without any additional mineral supplementation. Two hundred and forty (240) semen samples were collected from 40 bucks aged 11 months, through electro ejaculator method, processed and analysed for sperm quality parameters both at pre freeze and post-thaw stage. The semen samples were diluted in Tris egg yolk extender, cooled and equilibrated for 4 h at 5 °C, cryopreserved using programmable freezer (PLANER Kryo 360–1.7) and stored at −196 °C. The organic trace minerals (Zn, Cu and Zn + Cu) protected the spermatozoa against the cryoinjury and maintained higher post-thaw semen parameters except in high Zn group. Additional feeding of organic Cu and Zn to bucks had a protective role and resulted in higher sperm liveability, plasma membrane and acrosome integrities, motility and velocity and reduced oxidative stress in supplemented goats (P < 0.05). |
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| Keywords: | Zinc Copper Semen quality Cryopreservation Spermatozoa Bucks |
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