Cryoprotective effect of antifreeze polyamino-acid (Carboxylated Poly-l-Lysine) on bovine sperm: A technical note |
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| Affiliation: | 1. The United Graduate School of Veterinary Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, 753-8515, Japan;2. Kagoshima Prefectural Cattle Breeding Development Institute, 2200 Tsukino, So, 899-8212, Japan;3. Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan;4. BioVerde.Inc., 43-1 Minamimatsunoki-cho, Higashikujo, Minami-ku, Kyoto, 601-8023, Japan;5. Center for Fiber and Textile Science, Kyoto Institute of Technology (KIT), 105 Jibucho, Kyoto Fushimi-ku, Kyoto, 612-8374, Japan;1. Department of Obstetrics and Gynecology, c/-University Malaya Medical Centre, Faculty of Medicine, University of Malaya, Jalan Universiti, 50603 Kuala Lumpur, Malaysia;2. Fertility Center, Sunway Medical Centre, Jalan Lagoon Selatan, 47500 Kuala Lumpur, Malaysia;3. Department of Pathology, University Malaya Medical Centre, Faculty of Medicine, University of Malaya (UM), Jalan Universiti, 50603 Kuala Lumpur, Malaysia;4. Fertility Clinic, Hospital Kuala Lumpur, Jalan Pahang, 50586 Kuala Lumpur, Malaysia;1. Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Sagamihara 252-5201, Japan;2. Graduate School of Veterinary Science, Azabu University, Sagamihara 252-5201, Japan;3. Japan Advanced Institute of Science and Technology, Nomi 923-1291, Japan;4. Center for Fiber and Textile Science, Kyoto Institute of Technology, Matsugasaki, Kyoto 606-8585, Japan |
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| Abstract: | In this study, investigating Carboxylated Poly-l-Lysine (CPLL) for its effectiveness as a new cryoprotectant for bovine sperm is aimed. CPLL is an ampholytic polymer compound, has cryoprotective properties similar to those of anti-freeze protein. The cryopreservation medium used for control group consisted of 6.5% (v/v) glycerol, the cryopreservation medium used for experimental group consisted of 3.25% (v/v) glycerol + 0.5% (w/v) CPLL. There was no consequential difference in sperm motility parameter after thawing whereas there was huge distinction for sperm membrane integrity rate (control vs experimental; 49.6 vs 60.7%, P < 0.01). Conception rate of artificial insemination of experimental group was significantly higher than that of control group (79.0% vs 53.1%, P < 0.01). These results suggest CPLL has protected sperm membrane and leads to improve fertility. This is the first report using CPLL for bovine sperm cryopreservation, it is also expected CPLL can be applied to other animal species. |
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| Keywords: | Bovine Cryopreservation Glycerol Sperm |
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