Preparation of stearoyl lactic acid ester catalyzed by lipases from Rhizomucor miehei and porcine pancreas optimization using response surface methodology |
| |
| Authors: | K R Kiran N G Karanth S Divakar |
| |
| Institution: | (1) Department of Fermentation Technology and Bioengineering, Central Food Technological Research Institute, Mysore, 570 013, India e-mail: divakar@nicfos.ernet.in Tel.: +91-821-515792 Fax: +91-821-517233, IN |
| |
| Abstract: | The esterification reaction between stearic acid and lactic acid using Rhizomucor miehei lipase and porcine pancreas lipase was optimized for maximum esterification using response surface methodology. The formation
of the ester was found to depend on three parameters namely enzyme/substrate ratio, lactic acid (stearic acid) concentration
and incubation period. The maximum esterification predicted by theoretical equations for both lipases matched well with the
observed experimental values. In the case of R. miehei lipase, stearoyl lactic acid ester formation was found to increase with incubation period and lactic acid (stearic acid)
concentrations with maximum esterification of 26.9% at an enzyme/substrate (E/S) ratio of 125 g mol−1. In the case of porcine pancreas lipase, esterification showed a steady increase with increase in incubation period and lactic
acid (stearic acid) concentration independent of the E/S ratios employed. In the case of PPL, a maximum esterification of
18.9% was observed at an E/S ratio of 25 g mol−1 at a lactic acid (stearic acid) concentration of 0.09 M after an incubation period of 72 h.
Received: 12 February 1999 / Received revision: 31 May 1999 / Accepted: 4 June 1999 |
| |
| Keywords: | |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
