An Improved Method for Preparing the Chromosomes of Pines and other Gymnosperms |
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| Authors: | Brian G. Murray Blair J. Davies |
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| Affiliation: | a School of Biological Sciences, The University of Auckland, Private Bag, Auckland, New Zealand |
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| Abstract: | A simple technic is described to produce well spread gymnosperm chromosomes. Root tip meristems are digested with a pectinase:cellulase mixture to produce a cell suspension which then is squashed to yield flat, well spread chromosome complements that can be stained or used for in situ hybridization. |
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| Keywords: | cellulase chromosome spreading enzyme digestion gymnosperms pectinase |
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