Development and evaluation of different normalization strategies for gene expression studies in Candida albicans biofilms by real-time PCR |
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Authors: | Heleen Nailis Tom Coenye Filip Van Nieuwerburgh Dieter Deforce Hans J Nelis |
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Institution: | (1) Laboratory for Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium;(2) Laboratory for Pharmaceutical Biotechnology, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium |
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Abstract: | Background
Candida albicans biofilms are commonly found on indwelling medical devices. However, the molecular basis of biofilm formation and development
is not completely understood. Expression analysis of genes potentially involved in these processes, such as the ALS (Agglutinine Like Sequence) gene family can be performed using quantitative PCR (qPCR). In the present study, we investigated
the expression stability of eight housekeeping genes potentially useful as reference genes to study gene expression in Candida albicans (C. albicans) biofilms, using the geNorm Visual Basic Application (VBA) for Microsoft Excel. To validate our normalization strategies
we determined differences in ALS1 and ALS3 expression levels between C. albicans biofilm cells and their planktonic counterparts. |
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