Preparation of antibodies to roridin A and their applications |
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| Authors: | Birkin A A Kononenko G P Zorian V G Soboleva N A Zotova E V |
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| Institution: | Russian Institute of Veterinary Sanitary, Hygiene, and Ecology, Russian Academy of Agricultural Sciences, Moscow, Russia. |
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| Abstract: | Polyclonal rabbit antibodies against a conjugate synthesized through condensing BSA and disubstituted roridin A hemisuccinate allowed roridin A to be determined in solutions at a sensitivity of 0.2 ng/ml. The cross-reactivity of structural analogues--roridin A, verrucarin, and verrucarol--amounted to 100, 2.5, and 0.03%, respectively. The data showed that these antibodies determine roridin A in an indirect heterogeneous enzyme immunoassay in cereal straw samples at a sensitivity of 20 micrograms/kg. |
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