Determination of Abscisic Acid in Pinus densiflora by Selected Ion Monitoring |
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Authors: | Funada R Sugiyama T Kubo T Fushitani M |
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Affiliation: | Department of Forest Products, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183, Japan. |
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Abstract: | Abscisic acid (ABA) in the stem of akamatsu (Pinus densiflora) was identified and quantified by gas chromatography-mass spectrometry-selected ion monitoring using hexadeuterated ABA as an internal standard. tert-Butyldimethylsilyl ester was used as a derivative of ABA. This derivative had high sensitivity and selectivity for ABA determination. ABA concentrations in cambial region scrapings were independent of the cessation of cambial activity. |
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