| 台湾牛樟总RNA提取方法的建立 |
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| 引用本文： | 孟红岩,郭莺,林文珍,汪文华,刘黎卿.台湾牛樟总RNA提取方法的建立[J].亚热带植物科学,2016,45(1):53-56. |
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| 作者姓名： | 孟红岩 郭莺 林文珍 汪文华 刘黎卿 |
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| 作者单位： | (福建省亚热带植物研究所，福建省亚热带植物生理生化重点实验室，福建 厦门 361006) |
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| 基金项目： | 厦门市对台科技合作项目(3502Z20131150) |
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| 摘 要： | 以牛樟Cinnamomum kanehirae根、茎、叶为材料，在RNAprep Pure Plant Kit (Polysaccharides & Polyphenolics-rich)方法的基础上进行改良，建立台湾牛樟总RNA的提取方法。经琼脂糖凝胶电泳、Nanodrop和Aglient 2100检测发现，牛樟根、茎、叶所得RNA的28S和18S比值介于1.7～2.1之间，RNA完整性较好，浓度均在50 ng·μL-1以上，RIN值均大于7。该方法提取的RNA纯度、浓度和完整性均合格，可满足后续分子生物学实验要求。
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| 关 键 词： | 牛樟 RNA提取 |
| 收稿时间： | 2016-01-15 |
| 修稿时间： | 2016-04-17 |
A Method for Extraction of Total RNA from Cinnamomum kanehirae |
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| MENG Hong-yan,GUO Ying,LIN Wen-zhen,WANG Wen-hua,LIU Li-qing.A Method for Extraction of Total RNA from Cinnamomum kanehirae[J].Subtropical Plant Science,2016,45(1):53-56. |
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| Authors: | MENG Hong-yan GUO Ying LIN Wen-zhen WANG Wen-hua LIU Li-qing |
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| Abstract: | In this study, total RNA extraction of roots, stems, and leaves from Cinnamomum kanehirae was carried out. Electrophoresis, Nanodrop and Aglient 2100 analysis indicated that the value of 28S/18S was 1.7 to 2.1, RNA?integrity number was higher than 7, and RNA concentration was higher than 50 ng·μL-1, indicating that RNA was intact. In summary, the study showed that the purity, concentration and?integrity of the isolated RNA were qualified, and could be used for subsequent molecular biology studies. |
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| Keywords: | Cinnamomum kanehirae RNA extraction |
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