Genetic analysis of helper virus-specific selective amplification of cucumber mosaic virus satellite RNAs |
| |
Authors: | Marilyn J Roossinck Peter Palukaitis |
| |
Institution: | (1) Plant Biology Division, The S.R. Noble Foundation, P.O. Box 2180, 73402 Ardmore, OK, USA;(2) Plant Pathology Department, Cornell University, 14853 Ithaca, NY, USA |
| |
Abstract: | Satellite RNAs (sat-RNAs) are small molecular parasites associated with a number of plant RNA viruses. The cucumber mosaic virus (CMV) sat-RNAs are ca. 335 nucleotides and have evolved to produce a large number of closely related sat-RNAs. Different cucumoviruses can act as helper viruses in the amplification of CMV sat-RNAs. We have found that different helper viruses show a preference for a particular sat-RNA in a mixed infection. In this study the specificity of WL47 sat-RNA amplification by LS-CMV and of D4 sat-RNA amplification by tomato aspermy virus in mixed infections was examined. Recombinant cDNA clones of D4 sat-RNA and WL47 sat-RNA were used to map the sat-RNA sequences responsible for the helper virus selection of a specific sat-RNA for amplification.Correspondence to: M.J. Roossinck |
| |
Keywords: | Cucumber mosaic virus Genetic mapping RNA viruses Satellite RNA Selection |
本文献已被 SpringerLink 等数据库收录! |