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Flow-cytometric analysis of mitochondria-associated fluorescence in young and old human fibroblasts
Authors:Andrew O Martinez  Annette Vigil  Juan C Vila
Institution:1. Medical School, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK;2. Birmingham Centre for Women''s and Children''s Health, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK;3. School of Health and Population Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK;4. Sanyu Africa Research Institute (SAfRI), University of Liverpool, UK and Uganda;5. Department of General Practice and Rural Health, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand;1. Department of Respiratory and Critical Medicine, The First Affiliated Hospital of Soochow University, Suzhou, China;2. Institute of Systems Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China;3. Suzhou Institute of Systems Medicine, Suzhou Jiangsu, 215123 China
Abstract:Rhodamine 123 fluorescence in populations of young and old human fibroblasts was analysed and quantified with a fluorescence-activated cell sorter (FACS). Old fibroblasts exhibited a higher mean relative fluorescence than young fibroblasts. Moreover, two distinct subpopulations were evident in the fluorescence distributions of old cells--but not in those of young cells.
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