Semiautomated metabolic staining assay for Bacillus cereus emetic toxin |
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| Authors: | Finlay W J Logan N A Sutherland A D |
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| Institution: | School of Biological and Biomedical Sciences, Glasgow Caledonian University, Glasgow G4 OBA, United Kingdom. |
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| Abstract: | This paper describes a specific, sensitive, semiautomated, and quantitative Hep-2 cell culture-based 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay for Bacillus cereus emetic toxin. Of nine Bacillus, Brevibacillus, and Paenibacillus species assessed for emetic toxin production, only B. cereus was cytotoxic. |
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