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Respirometry increases cortisol levels in rainbow trout Oncorhynchus mykiss: implications for measurements of metabolic rate |
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| Authors: | L. Murray M. D. Rennie J. C. Svendsen E. C. Enders |
| Affiliation: | 1. University of Manitoba, Department of Biological Sciences, Winnipeg, MB, Canada;2. Lakehead University, Department of Biology, Thunder Bay, ON, Canada;3. IISD‐Experimental Lakes Area, Winnipeg, MB, Canada;4. Technical University of Denmark, Charlottenlund, Denmark;5. Fisheries & Oceans Canada, Freshwater Institute, Winnipeg, MB, R3T 2N6, Canada |
| Abstract: | This study aimed to assess the extent to which chasing, handling and confining Oncorhynchus mykiss to a small respirometer chamber during respirometric experiments is stressful and affects metabolic measurements. The study observed increased cortisol levels in animals tested using a chase protocol and subsequent intermittent‐flow respirometry, suggesting that this procedural treatment may stress animals. |
| Keywords: | fishes metabolism salmonid standard metabolic rate static respirometry stress |