Somatic embryogenesis and plant regeneration from undeveloped ovules and stigma/style explants of sweet orange navel group [ Citrus sinensis (L.) Osb.] |
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Authors: | Carimi Francesco Tortorici Maria Concetta De Pasquale Fabio Crescimanno Francesco Giulio |
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Affiliation: | (1) Centro di Studio per il Miglioramento Genetico degli Agrumi, Consiglio Nazionale delle Ricerche c/o Facoltà di Agraria, Viale delle Scienze 11, 1-90128 Palermo, Italy (Fax |
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Abstract: | Somatic embryogenesis was induced and plant regeneration was obtained in 11 different genotypes of sweet orange navel group [Citrus sinensis (L.) Osb.] from cultures of stigma/style explants and undeveloped ovules. Explants were cultured on 3 different modifications of Murashige and Skoog medium: 500 mg l-1 malt extract; 500 mg l-1 malt extract and 4.6 μM kinetin; and 500 mg l-1 malt extract and 13.3 μM 6-benzylaminopurine. Sucrose (146 mM) was used as carbon source. Somatic embryogenesis occurred 1–3 months after culture initiation from undeveloped ovule and stigma/style cultures of all the genotypes tested. Somatic embryos developed into plantlets with a high frequency (74%) after transfer to Murashige and Skoog medium supplemented with 146 mM sucrose and 500 mg l-1 malt extract. Plants were successfully transferred to soil. This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | cell suspension culture embryogenic callus nucellar embryos plant regeneration tissue culture |
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