| 巴斯德毕赤酵母表达外源蛋白的优化策略 |
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| 引用本文： | 樊英,李天保,杨秀生,王建华,滕达. 巴斯德毕赤酵母表达外源蛋白的优化策略[J]. 生物技术通报, 2010, 0(12) |
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| 作者姓名： | 樊英 李天保 杨秀生 王建华 滕达 |
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| 作者单位： | 1. 山东省海水养殖研究所,山东省海水养殖病害防治重点实验室,青岛,266002
2. 中国农业科学院饲料研究所,北京,100081 |
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| 摘 要： | 巴斯德毕赤酵母(Pichia pastoris)表达系统已成为外源蛋白最理想的表达系统之一,诸多的优点体现了其广泛的研究价值和应用价值。综述了P.pastoris表达外源蛋白时在载体选择与利用、外源基因改造、翻译后修饰及表达稳定性等方面的优化策略,以加速其应用。
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| 关 键 词： | 巴斯德毕赤酵母 外源蛋白 优化表达 |
Strategies for Expression Optimization of Heterologous Proteins in Pichia pastoris |
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| Fan Ying,Li Tianbao,Yang Xiusheng,Wang Jianhua,Teng Da. Strategies for Expression Optimization of Heterologous Proteins in Pichia pastoris[J]. Biotechnology Bulletin, 2010, 0(12) |
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| Authors: | Fan Ying Li Tianbao Yang Xiusheng Wang Jianhua Teng Da |
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| Affiliation: | Fan Ying1 Li Tianbao1 Yang Xiusheng1 Wang Jianhua2 Teng Da2(Shandong Province Mariculture Research Institute,Shandong Province Key Laboratory for Disease Control ofMariculture,Qingdao 266002,2Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081) |
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| Abstract: | Pichia pastoris has been developed into one of highly successful systems for expression of various heterologous proteins,many advantages of which reveal wide values in research and application.The article summarized those factors and their optimization that influence expression of heterologous proteins in Pichia pastoris,including the choice and utilization of vectors,modifying heterologous genes,posttranslational modification and expression stability,thus being able to accelerate its application. |
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| Keywords: | Pichia pastoris Heterologous proteins Optimization expression |
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