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A scanning electron microscopic study of the appearance and localization of cell surface material during amphibian gastrulation.
Authors:D Moran  W E Mouradian
Affiliation:1. Department of Biology, State University of New York, New Paltz, New York, 12561 USA;2. The Orthopedic Research Laboratory and The International Institute for the Study of Human Reproduction, Columbia College of Physicians and Surgeons, New York, New York 10032 USA
Abstract:Cell surface material (CSM) has been implicated in a wide variety of morphogenetic processes. We describe here its discrete appearance at the blastoporal region throughout gastrulation in the salamander Ambystoma mexicanum. The CSM becomes visible in the scanning electron microscope after treatment with Alcian blue and lanthanum nitrate which reportedly attests to the presence of mucopolysaccharides. The possible importance of this CSM is underscored by its correlative appearance on tissue undergoing morphogenetic movement and induction.
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