24-表油菜素内酯对盐藻细胞分裂的促进作用及与Ca2+和钙调素的关系

外加２４表油菜素内酯（２４ｅｐｉＢＬ） 无论在光下或暗中均可促进盐藻细胞分裂数的增加，激动素只在光下具有这种作用。外界Ｃａ２＋ 浓度升高时，２４ｅｐｉＢＬ 促进细胞分裂的效果更为明显，而ＥＧＴＡ 可以抑制２４ｅｐｉＢＬ引起的促进作用。Ｖｅｒａｐａｍｉｌ、Ｗ７ 、环己酰亚胺均可抑制盐藻细胞分裂。Ｃａ２ ＋ 载体Ａ２３１８７ 在低浓度（０ ．２５ μｍｏｌ／Ｌ） 时具有促进分裂的作用。可以认为２４ｅｐｉＢＬ对低等单细胞藻类具有生理作用，并且，其促进盐藻细胞分裂的机制与激动素是不同的，它不仅与Ｃａ２ ＋ 有关，而且与钙调素也有较密切的关系。

24 epibrassinolide Promoting the Cell Division of Dunaliella salina Relation to Ca~(2 ) and Calmodulin

epibrassinolide was found to promote the cell division of Dunaliella salina,at a concentration as low as 0.005 mg/L, with an optimal concentration at about 0.5 mg/L(Fig.1). In contrast to kinetin,which is effective only in light, 24 epiBL could promote cell division both in light and in the dark(Fig.3). With Ca 2 , EGTA, A 23187 , and Verapamil we found that the promoting of cell division by 24 epiBL was related to Ca 2 and Ca 2 channel. The depression effect of W 7 indicates that the cell division of Dunaliella salina relates to calmodulin (CaM) (Fig.9). These results may indicate that 24 epiBL could promote cell division of Dunaliella salina and has a different way from kinetin. Moreover, 24 epiBL has its effect on the cell division through increasing cytoplasm Ca 2 concentration.The results also imply that 24 epiBL regulated cell division through its effect on protein synthesis.