Validated high performance liquid chromatography-UV detection method for the determination of daptomycin in human plasma |
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Authors: | Martens-Lobenhoffer Jens Kielstein Jan T Oye Catrin Bode-Böger Stefanie M |
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Affiliation: | Institute of Clinical Pharmacology, Otto-von-Guericke University, Leipziger Str. 44, Magdeburg 39120, Germany. jens.martens-lobenhoffer@med.ovgu.de |
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Abstract: | Daptomycin is the first approved member of the new class cyclic lipopeptide antibiotic drugs, effective against a broad spectrum of Gram-positive bacteria. Here we present an HPLC method with UV detection capable to obtain pharmacokinetic data of daptomycin in human plasma, exemplarily shown in a critically ill patient with acute renal failure undergoing extended daily dialysis. Sample preparation consists only of protein precipitation with methanol. Chromatographic separation was achieved on a Zorbax Eclipse XDB-C8 column and daptomycin was detected at 224 nm. The calibration function was linear over the range from 3.5 to 350 microg/ml. The relative standard deviations were <2% in the intra-day and <6% in the inter-day measurements. The accuracy was always better than 7%. Daptomycin was stable in aqueous solutions for 2 months frozen at-20 degrees C. However, in plasma frozen at -20 degrees C a loss of 25% in 1 month was observed. |
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