Optimization of biosurfactant production using waste from biodiesel industry in a new membrane assisted bioreactor |
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Authors: | Alyson G. Pereira Graziela J. Pacheco Luiz F. Tavares Bianca C. Neves Frederico de A. Kronemberger Rodrigo S. Reis Denise M.G. Freire |
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Affiliation: | 1. Cellular Biology Department, University of Brasilia, Brasilia, DF 70100-000, Brazil;2. Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro RJ 21945-970, Brazil;3. Instituto Alberto Luiz Coimbra de Pós-Graduação e Pesquisa de Engenharia, Federal University of Rio de Janeiro, Rio de Janeiro RJ 21945-970, Brazil;4. School of Molecular Biosciences, University of Sydney, New South Wales 2006, Australia |
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Abstract: | The main byproduct of biodiesel production is glycerol. Here, crude glycerol – byproduct of biodiesel industry – was evaluated as sole carbon source in rhamnolipids production by Pseudomonas aeruginosa. The optimal concentration of crude glycerol and sodium nitrate was assessed using response surface methodology, resulting in about 40–50 mg/L.h of rhamnolipids, which was about four times higher than previously reported in the literature. Fermentation parameters were similar to those observed with commercial glycerol as sole carbon source. The optimized medium was suitable for production using simple (22.9 mg/L.h) and fed-batch (32.4 mg/L.h) fermentation in oxygen-controlled bioreactor without foaming formation. Composition and relative abundance of rhamnolipid congeners showed that crude glycerol had little effect on metabolic pathways involved in their production. CMC values were approximately 130 mg/L and 230–260 mg/L for rhamnolipids from crude and commercial glycerol fermentation, respectively, which were about 2–6 times lower than CMC values of synthetic surfactants. |
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Keywords: | Factorial design Surface response analysis Rhamnolipid Bioreactor Crude glycerol |
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