Impact of Growth Hormone (GH) Deficiency and GH Replacement upon Thymus Function in Adult Patients |
| |
| Authors: | Gabriel Morrhaye Hamid Kermani Jean-Jacques Legros Frederic Baron Yves Beguin Michel Moutschen Remi Cheynier Henri J. Martens Vincent Geenen |
| |
| Affiliation: | 1. University of Liege Center of Immunology, Laboratory of Immunoendocrinology, Institute of Pathology CHU-B23, Liege-Sart Tilman, Belgium.; 2. University of Liege, Division of Hematology, CHU-B35, Liege-Sart Tilman, Belgium.; 3. University of Liege, Division of Immunodeficiencies and Infectious Diseases, CHU-B35, Liege-Sart Tilman, Belgium.; 4. Institut Pasteur, Département de Virologie, Paris, France.;New York University School of Medicine, United States of America |
| |
| Abstract: | BackgroundDespite age-related adipose involution, T cell generation in the thymus (thymopoiesis) is maintained beyond puberty in adults. In rodents, growth hormone (GH), insulin-like growth factor-1 (IGF-1), and GH secretagogues reverse age-related changes in thymus cytoarchitecture and increase thymopoiesis. GH administration also enhances thymic mass and function in HIV-infected patients. Until now, thymic function has not been investigated in adult GH deficiency (AGHD). The objective of this clinical study was to evaluate thymic function in AGHD, as well as the repercussion upon thymopoiesis of GH treatment for restoration of GH/IGF-1 physiological levels.Methodology/Principal FindingsTwenty-two patients with documented AGHD were enrolled in this study. The following parameters were measured: plasma IGF-1 concentrations, signal-joint T-cell receptor excision circle (sjTREC) frequency, and sj/β TREC ratio. Analyses were performed at three time points: firstly on GH treatment at maintenance dose, secondly one month after GH withdrawal, and thirdly one month after GH resumption. After 1-month interruption of GH treatment, both plasma IGF-1 concentrations and sjTREC frequency were decreased (p<0.001). Decreases in IGF-1 and sjTREC levels were correlated (r = 0.61, p<0.01). There was also a decrease in intrathymic T cell proliferation as indicated by the reduced sj/β TREC ratio (p<0.01). One month after reintroduction of GH treatment, IGF-1 concentration and sjTREC frequency regained a level equivalent to the one before GH withdrawal. The sj/β TREC ratio also increased with GH resumption, but did not return to the level measured before GH withdrawal.ConclusionsIn patients with AGHD under GH treatment, GH withdrawal decreases thymic T cell output, as well as intrathymic T cell proliferation. These parameters of thymus function are completely or partially restored one month after GH resumption. These data indicate that the functional integrity of the somatotrope GH/IGF-1 axis is important for the maintenance of a normal thymus function in human adults.Trial RegistrationClinicalTrials.gov NTC00601419 |
| |
| Keywords: | |
|
|
