A novel thermostable cellulase free xylanase stable in broad range of pH from Streptomyces sp. CS428 |
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Authors: | Pradeep GC Yun Hee Choi Yoon Seok Choi Chi Nam Seong Seung Sik Cho Hyo Jeong Lee Jin Cheol Yoo |
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Institution: | 1. Department of Pharmacy, Chosun University, Gwangju 501-759, Republic of Korea;2. Department of Biology, College of Natural Sciences, Sunchon National University, Sunchon, Jeonnam 540-742, Republic of Korea;3. Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam 534-729, Republic of Korea;4. Department of Alternative Medicine, Gwangju University, Gwangju 503-703, Republic of Korea |
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Abstract: | A cellulase free thermostable xylanase from Streptomyces sp. CS428 was isolated from a Korean soil sample, purified by single-step chromatography, and biochemically characterized. The extracellular xylanase was purified 26 fold with a 55% yield by CM Trisacryl cation exchange chromatography. The molecular mass of the enzyme (Xyn428) was approximately 37 kDa. Xyn428 was found to be stable over a broad pH range (4 to ~13.6) and to 50 °C and have an optimum temperature of 80 °C. Xyn428 had Km and Vmax values of 102.3 ± 1.2 mg/mL and 3225.4 ± 15 mmol/min mg, respectively, when beechwood xylan was used as substrate. N-terminal sequence of Xyn428 was INRTDHNENSYLEIHNNEAR. CS428 was grown on different agro waste xylan and produced 4197.1 U/mL of xylanase activity in 36 h of cultivation in wheat bran without supplements. Xyn428 activity was inhibited by Tris salt at concentrations above 20 mM, and produced xylose and xylobiose as major products. It was found to degrade agro waste materials by small unit of enzyme (20 U/g) as shown by electron microscopy. As being simple in purification, thermo tolerant, pH stability in broad range and ability to produce xylooligosaccharides show that Xyn428 has potential applications in industries as a biobleaching agent and for xylooligosaccharides production. |
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Keywords: | Xylanase Single-step purification Agro-waste Xylooligosaccharides |
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