Enzyme-linked immunosorbent assay for the diagnosis of Wuchereria bancrofti infection using urine samples and its application in Bangladesh |
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| Authors: | Mohammad Sohel Samad Makoto Itoh Kazuhiko Moji Moazzem Hossain Dinesh Mondal Mohammad Shafiul Alam Eisaku Kimura |
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| Institution: | 1. Department of Infection and Immunology, Aichi Medical University School of Medicine, 1-1 Yazakokarimata, Nagakute, Aichi 480-1195, Japan;2. Research Institute for Humanity and Nature (RIHN: Chikyu-ken), 457-4 Motoyama, Kamigamo, Kita-ku, Kyoto 603-8047, Japan;3. Institute of Allergy & Clinical Immunology of Bangladesh (IACIB), Green Super Market, Green Road, Dhaka 1205, Bangladesh;4. International Center for Diarrheal Disease and Research, Bangladesh (icddr,b) 68, Shahid Tajuddin Ahmed Sharani, Mohakhali, Dhaka 1212, Bangladesh |
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| Abstract: | In Sri Lanka, urine ELISA showed high sensitivity and specificity in detecting filaria-specific IgG4. It also produced much higher positive rates than antigen tests in prevalence studies with young children. In this study, we have confirmed the usefulness of urine ELISA in the field of Bangladesh. The ELISA detected 89 of 105 (85%) ICT antigen test positive subjects in endemic areas. With both ICT and microfilaria positives, the sensitivity was 97% (30/31). All of 104 ICT negative people in a non-endemic area were ELISA negative (100% specificity). In a prevalence study with 319 young children (5–10 years) from a low endemic area after five rounds of MDA, seven (2.2%) were detected by the present urine test, but only one (0.3%) by ICT (P = 0.075). The satisfactorily high sensitivity, 100% specificity and effective case detection among young ages along with scope for analyzing the titers will indicate urine ELISA to be an effective tool in the post-MDA surveys to confirm elimination or to detect resurgence in Bangladesh. |
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