Physicochemical characterization of a thermostable glycoprotein bioemulsifier from Solibacillus silvestris AM1 |
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Authors: | A.R. Markande S.R. Acharya A.S. Nerurkar |
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Affiliation: | 1. Department of Microbiology and Biotechnology Centre, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara 390002, Gujarat, India;2. Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara 390002, Gujarat, India |
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Abstract: | A novel estuarine bacterial strain, Solibacillus silvestris AM1, was found to produce an extracellular, multimeric glycoprotein bioemulsifier, termed AM1, with a MW of 200 kDa and containing 30 kDa monomeric subunits. The bioemulsifier contained 3.6% of the minor carbohydrate components galactose and ribose/xylose. LC/MS-MS of the 30 kDa subunit revealed its homology with a flagellin-like protein arranged in the form of fibers, as shown by transmission electron micrographs. This is the first report of a flagellin-like protein that exhibits bioemulsifier activity being produced from a member of the Solibacillus genus. Bioemulsifier AM1 has a high emulsification index of 62.5% with 10?2 critical micellar dilution. It was found to be thermostable and active in the pH 5–9 and 0–5 M NaCl ranges. Moreover, AM1 formed stable emulsions with a broad range of solvents, including aliphatics, aromatic hydrocarbons and oils, performing better than the well-known bioemulsifier emulsan. Emulsions formed with trichlorobenzene and paraffin oil have a pseudoplastic non-Newtonian rheological property, as observed by particle size and shear stress analysis. AM1, an eco-friendly bioemulsifier, formed stable emulsions in varied physical conditions, and these attributes may prove to be advantageous in cosmetic, pharmaceutical and environmental applications. |
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Keywords: | Glycoprotein bioemulsifier Flagellin Thermostability LC/MS-MS Emulsion stability |
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