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A sensitive and specific PCR assay for the detection of Baylisascaris schroederi eggs in giant panda feces
Authors:Ning Wang  De-Sheng Li  Xuan Zhou  Yue Xie  Yi-Nan Liang  Cheng-Dong Wang  Hua Yu  Shi-Jie Chen  Yu-Bo Yan  Xiao-Bin Gu  Shu-Xian Wang  Xue-Rong Peng  Guang-You Yang
Institution:1. Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya''an 625014, China;2. China Conservation and Research Center for the Giant Panda, Wolong 623006, China;3. Sichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu 610041, China;4. Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya''an 625014, China
Abstract:Baylisascaris schroederi is one of the most common intestinal nematodes in giant pandas. It can cause severe baylisascariasis which is highly infectious in its natural hosts. A rapid and reliable diagnosis of parasite infections is crucial to protect giant pandas, as well as for environmental monitoring and disease surveillance. Here, we established a specific PCR assay for B. schroederi detection which was targeting a 331-bp long fragment of the mitochondrial cytochrome c oxidase subunit II (COII) gene. Fifty fresh fecal samples collected from captive giant pandas were tested by the established PCR assay and the traditional flotation technique. DNA extracted from a single B. schroederi egg could be successfully amplified, while no cross-reactivity was found with DNA from Ancylostoma caninum eggs. The detection rate of the PCR assay was 68%, which was higher than that of the traditional egg flotation (46%). Our findings demonstrated that the PCR assay is sensitive and specific for the detection and identification of B. schroederi eggs. Therefore, it could become a useful tool for the investigation of B. schroederi infections in giant pandas.
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