A sensitive and specific PCR assay for the detection of Baylisascaris schroederi eggs in giant panda feces |
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Authors: | Ning Wang De-Sheng Li Xuan Zhou Yue Xie Yi-Nan Liang Cheng-Dong Wang Hua Yu Shi-Jie Chen Yu-Bo Yan Xiao-Bin Gu Shu-Xian Wang Xue-Rong Peng Guang-You Yang |
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Institution: | 1. Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya''an 625014, China;2. China Conservation and Research Center for the Giant Panda, Wolong 623006, China;3. Sichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu 610041, China;4. Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya''an 625014, China |
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Abstract: | Baylisascaris schroederi is one of the most common intestinal nematodes in giant pandas. It can cause severe baylisascariasis which is highly infectious in its natural hosts. A rapid and reliable diagnosis of parasite infections is crucial to protect giant pandas, as well as for environmental monitoring and disease surveillance. Here, we established a specific PCR assay for B. schroederi detection which was targeting a 331-bp long fragment of the mitochondrial cytochrome c oxidase subunit II (COII) gene. Fifty fresh fecal samples collected from captive giant pandas were tested by the established PCR assay and the traditional flotation technique. DNA extracted from a single B. schroederi egg could be successfully amplified, while no cross-reactivity was found with DNA from Ancylostoma caninum eggs. The detection rate of the PCR assay was 68%, which was higher than that of the traditional egg flotation (46%). Our findings demonstrated that the PCR assay is sensitive and specific for the detection and identification of B. schroederi eggs. Therefore, it could become a useful tool for the investigation of B. schroederi infections in giant pandas. |
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