Prion Strain Discrimination Based on Rapid In Vivo Amplification and Analysis by the Cell Panel Assay |
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| Authors: | Yervand Eduard Karapetyan Paula Saá Sukhvir Paul Mahal Gian Franco Sferrazza Alexandra Sherman Nicole Salès Charles Weissmann Corinne Ida Lasmézas |
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| Affiliation: | Department of Infectology, The Scripps Research Institute, Scripps Florida, Jupiter, Florida, United States of America.;Massachusetts Institute of Technology, United States of America |
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| Abstract: | Prion strain identification has been hitherto achieved using time-consuming incubation time determinations in one or more mouse lines and elaborate neuropathological assessment. In the present work, we make a detailed study of the properties of PrP-overproducing Tga20 mice. We show that in these mice the four prion strains examined are rapidly and faithfully amplified and can subsequently be discriminated by a cell-based procedure, the Cell Panel Assay. |
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