Preparation,isolation and identification of iron-chelating peptides derived from Alaska pollock skin |
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Authors: | Lidong Guo Hu Hou Bafang Li Zhaohui Zhang Shanshan Wang Xue Zhao |
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Institution: | College of Food Science and Engineering, Ocean University of China, No. 5, Yushan Road, Qingdao, Shandong Province 266003, PR China |
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Abstract: | Alaska pollock skin as aquatic by-product was usually discarded in food processing. Iron deficiency has been a nutritional problem for a long period. In this study, Alaska pollock skin was used to generate iron-chelating peptides after being treated by commercial enzymes. Sequential chromatography, including immobilized metal affinity chromatography (IMAC) and reversed phase high performance liquid chromatography (RP-HPLC), was used for capturing and purifying iron-chelating peptides. One tripeptide, with high iron-chelating activity, was obtained and identified by electrospray ionization tandem mass spectrometry (LC–ESI-MS/MS). The amino acid sequence of the iron-chelating peptide was identified to be Ser-Cys-His (MW: 345 Da). These results show that Alaska pollock skin could be utilized to generate iron-chelating peptides as an iron supplement in functional food industry. |
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Keywords: | Iron-chelating peptides Bioavailability Iron Ser-Cys-His Immobilized metal affinity chromatography (IMAC) Tandem mass spectrum (MS/MS) |
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