Influence of DNA-repair gene variants on the micronucleus frequency in thyroid cancer patients |
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| Authors: | WA García-Quispes S Pastor P Galofré F Biarnés J Castell A Velázquez R Marcos |
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| Institution: | 1. Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain;2. CIBER Epidemiología y Salud Pública, Instituto de Salud Carlos III, Spain;3. Servei de Medicina Nuclear, Hospitals Universitaris Vall d’Hebron, Barcelona, Spain;4. Servei de Medicina Nuclear, Hospital Josep Trueta, Girona, Spain;5. Unitat d’Endocrinología, Hospital Josep Trueta, Girona, Spain |
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| Abstract: | The role of different DNA-repair genes (OGG1, XRCC1, XRCC2 and XRCC3) on both the spontaneous and the induced frequency of micronuclei (MN) has been studied in the lymphocytes of a group of 114 patients with differentiated thyroid cancer (DTC). Induction of MN was achieved by treatment of the lymphocytes with 0.5 Gy of gamma-radiation.The selected genes are involved in base-excision repair (BER) (OGG1, Ser326Cys; XRCC1, Arg280His and Arg399Gln), and in homologous recombination repair (HRR) (XRCC2, Arg188His and XRCC3, IVS5-14G). Genotyping was carried out by use of the iPLEX (Sequenom) technique.Results indicate that only the OGG1-Ser326Cys polymorphism was able to modulate the MN frequency. This effect was only observed in the spontaneous MN frequency (P = 0.016), but not in the MN frequency induced after irradiation. In addition, a strong correlation was observed between spontaneous and induced MN frequency, which would suggest an underlying genetic background. |
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