Rapid confirmation of Clostridium perfringens by using chromogenic and fluorogenic substrates |
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| Authors: | Adcock P W Saint C P |
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| Institution: | Microbiology Unit, Australian Water Quality Centre, SA Water Corporation, Salisbury, South Australia 5108, Australia. |
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| Abstract: | The use of 4-methylumbelliferyl phosphate (MUP) and ortho-nitrophenyl-beta-D-galactopyranoside (ONPG) for the identification of Clostridium perfringens was investigated. A liquid assay containing both MUP and ONPG was a highly specific alternative method for C. perfringens confirmation, reducing incubation time from 48 to only 4 h. The assay solution is easy to prepare, does not require anaerobic conditions for use, and has an extended shelf life. |
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