H+/e- stoichiometry of mitochondrial cytochrome complexes reconstituted in liposomes. Rate-dependent changes of the stoichiometry in the cytochrome c oxidase vesicles. |
| |
| Authors: | N Capitanio G Capitanio E De Nitto G Villani S Papa |
| |
| Affiliation: | Institute of Medical Biochemistry and Chemistry, Faculty of Medicine, University of Bari, Italy. |
| |
| Abstract: | The H+/e- stoichiometry of protonmotive cytochrome c oxidase, isolated from bovine heart mitochondria and reconstituted in liposomes, has been determined by making use of direct spectrophotometric measurements of the initial rates of e- flow and H+ translocation. It is shown that the ----H+/e- ratio for redox-linked proton ejection by the oxidase varies from around 0 to a maximum of 1 as a function of the rate of overall electron flow in the complex. |
| |
| Keywords: | |
|
|
