Protein-lipid interactions in reconstituted high density lipoproteins: apolipoprotein and cholesterol influence. |
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Authors: | A D Dergunov G E Dobretsov S Visvikis G Siest |
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Affiliation: | National Research Centre for Preventive Medicine, 10, Petroverigsky Street, 101953 Moscow, Russia. dergunov@img.ras.ru |
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Abstract: | Two fluorescent probes-cis- and trans-parinaric acids were used to study the dimensions, lipid dynamics and apolipoprotein location in the reconstituted discoidal high density lipoproteins (rHDL). The rHDL particles made from apolipoprotein A-I (apoA-I), dipalmitoylphosphatidylcholine (DPPC), with or without cholesterol (Chol) were compared with the analogous particles with two other apolipoproteins-apoE and apoA-II. The data obtained for apoA-I-containing rHDL were as follows: (1) the inclusion of 8 mol.% of cholesterol did not significantly change the particle dimensions (13+/-1 nm) or the mean distance between apoA-I and the disc axis; (2) the phospholipid domains-boundary lipid region in the close vicinity to apoA-I molecule and the remaining part of the bilayer-existed at temperatures both lower and above DPPC transition temperature T(t); (3) at T
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