Resolution of the conformational distribution and dynamics of a flexible molecule using frequency-domain fluorometry |
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| Authors: | J R Lakowicz J Ku?ba W Wiczk I Gryczynski H Szmacinski M L Johnson |
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| Affiliation: | University of Maryland, School of Medicine, Department of Biological Chemistry, Baltimore 21201. |
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| Abstract: | We report the first resolution of both the conformational distribution and end-to-end diffusion coefficient of a flexible molecule. This molecular information was recovered using only the donor intensity decay in a single solvent at a single viscosity, as observed by the technique of frequency-domain fluorometry. This technique can be extended to measurements of structural fluctuations of biological macromolecules. |
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