A modified mini-primer set for analyzing mitochondrial DNA control region sequences from highly degraded forensic samples |
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Authors: | Lee Hwan Young Kim Na Young Park Myung Jin Yang Woo Ick Shin Kyoung-Jin |
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Institution: | Department of Forensic Medicine, Yonsei University College of Medicine, Yonsei University, Seoul, Korea. |
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Abstract: | To facilitate the analysis of mitochondrial DNA (mtDNA) control region sequences from highly degraded skeletal remains, a modified mini-primer set was designed to overcome the limitations of the Armed Forces DNA Identification Laboratory (AFDIL) mini-primer set. This modified mini-primer set is less affected by nucleotide variability and PCR amplification conditions than the AFDIL mini-primer set, and was able to amplify the mtDNA sequences of 55-year-old skeletal remains with high efficiency, indicating that it is a useful tool for analyzing mtDNA control region sequences from highly degraded forensic samples. |
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