The fluorescent cholesterol analog dehydroergosterol induces liquid-ordered domains in model membranes |
| |
| Authors: | Olav Garvik Adam Cohen Simonsen Daniel Wüstner |
| |
| Institution: | a MEMPHYS Center for Biomembrane Physics, Department of Chemistry and Physics, University of Southern Denmark, DK-5230 Odense M, Denmark
b Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark |
| |
| Abstract: | The fluorescent sterol dehydroergosterol (DHE) is often used as a marker for cholesterol in cellular studies. We show by vesicle fluctuation analysis that DHE has a lower ability than cholesterol to stiffen lipid bilayers suggesting less efficient packing with phospholipid acyl chains. Despite this difference, we found by fluorescence and atomic force microscopy, that DHE induces liquid-ordered/-disordered coexistent domains in giant unilamellar vesicles (GUVs) and supported bilayers made of dipalmitoylphosphatidylcholine (DPPC), dioleylphosphatidylcholine (DOPC) and DHE or cholesterol. DHE-induced phases have a height difference of 0.9-1 nm similar as known for cholesterol-containing domains. DHE not only promotes formation of liquid-liquid immiscibility but also shows strong partition preference for the liquid-ordered phase further supporting its suitability as cholesterol probe. |
| |
| Keywords: | Sterol Membrane Fluorescence Bilayer Microscopy Intracellular transport |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
