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Spectroscopic characterization of DMPC/DOTAP cationic liposomes and their interactions with DNA and drugs |
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| Authors: | Jean-François Labbé René C-Gaudreault Michèle Auger |
| Institution: | a Département de Chimie, Centre de Recherche sur la Fonction, Structure et Ingénierie des Protéines, Centre de Recherche sur les Matériaux Avancés, Université Laval, Québec, Québec, Canada G1V 0A6 b Unité des Biotechnologies et de Bioingénierie, Centre de recherche, CHUQ, Hôpital Saint-François d’Assise, Université Laval, Canada G1L 3L5 c Faculté de médecine, Québec, Québec, Canada G1V 0A6 |
| Abstract: | Gene and synthetic drug-delivery vectors have been developed and characterized to treat several genetic diseases and cancers. Our study aims at characterizing cationic liposomes containing the zwitterionic phospholipid DMPC and the cationic lipid DOTAP as well as their interactions with two types of DNA and a new class of antineoplastic agents derived from arylchloroethylureas (CEU). Results obtained using FTIR spectroscopy as well as 31P and 2H NMR indicate that DMPC and DOTAP form cationic liposomes in a highly disordered fluid phase at a molar ratio of 1:1. In addition, the FTIR results indicate that the presence of DNA or CEUs within the liposomes does not significantly affect the conformational order of both the DMPC and DOTAP acyl chains. Our results therefore provide a detailed characterization of complexes between cationic liposomes and both DNA and drugs and indicate that these complexes are stable and fluid assemblies. |
| Keywords: | Cationic liposomes Membranes DNA Arylchloroethylureas FTIR NMR |
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