玉米离体根尖的多层滤纸床液体静止培养方法

设计建立了适于玉米根尖离体培养的多层滤纸床液体静止培养方法，培养的适宜体系为：1／4MS大量元素改良＋1／2MS微量元素＋IBA0.1-0.3mg/L，黑暗培养。该方法避免了传统液体培养通气状况不良的问题，玉米根的生长速度可达到1－2cm/d，分支和生长正常。该方法在控制条件下快速繁殖根系，成本低廉，简便易行，是根系发育和生理研究的理想实验体系。

Establishment of liquid culture method for in vitro maize root tip with filter-papers-bed and motionless

A new liquid culture using filter papers with motionless for corn root apical were established in this paper.The suitable culture medium included 1/4 enormous elements of MS,1/2 trace elements of MS and IBA 0.1~0.3 mg/L,and the roots should be grown under dark.The system gave the corn roots better aerate condition and made the roots grew faster than traditional liquid culture.Corn root grew by the rate of 1~2 cm/d,while developed and branched normally.The new method made in vitro cloning of roots fast and simply,and was proved to be the ideal experimental system for study on development and physiology of plant roots.