Embryogenesis and plant regeneration of hot pepper (<Emphasis Type="Italic">Capsicum annuum</Emphasis> L.) through isolated microspore culture |
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Authors: | Moonza Kim In-Chang Jang Jin-Ae Kim Eun-Joon Park Michung Yoon Youngwon Lee |
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Institution: | (1) Department of Life Sciences, Mokwon University, 800 Doan-dong Seo-gu, Taejon, 302-729, South Korea;(2) Department of Technology Marketing, Mokwon University, Taejon, South Korea |
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Abstract: | We report high frequencies of embryo production and plant regeneration through isolated microspore culture of hot pepper (Capsicum annuum L.). Microspores cultured in modified NLN medium (NLNS) divided and developed to embryos. Globular and heart-shaped embryos
were observed from 3 weeks after the beginning of culture, and many embryos reached the cotyledonary stage after 4 weeks of
culture. These cotyledonary embryos developed to plantlets after transfer to solid B5 basal medium. We also optimized conditions
for embryo production by varying the pretreatment media, the carbon sources, and culture densities. Heat shock treatment in
sucrose-starvation medium was more effective than in B5 medium. Direct comparisons of sucrose and maltose as carbon sources
clearly demonstrated the superiority of sucrose compared to maltose, with the highest frequency of embryo production being
obtained in 9% (w/v) sucrose. Microspore plating density was critical for efficient embryonic induction and development, with
an optimal plating density of 8 × 104–10 × 104/ml. Under our optimized culture conditions, we obtained over 54 embryos, and an average of 5.5 cotyledonary embryos when
10 × 104 microspores were grown on an individual plate. |
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Keywords: | Capsicum annuum L Haploid Hot pepper Isolated microspore culture Microspore embryogenesis |
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