A preliminary investigation of ursolic acid in cell suspension culture of Salvia officinalis |
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| Authors: | Bolta iga Bari evi Dea Bohanec Borut Andren ek Samo |
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| Affiliation: | (1) Biotechnical Faculty, University of Ljubljana, 1111 Ljubljana, Slovenia;(2) Department for Food Chemistry, National Institute of Chemistry, 1000 Ljubljana, Slovenia |
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| Abstract: | Callus and suspension cultures of two genotypes and two morphological forms (friable and compact) were established on MS medium supplemented with 10.47 μM NAA and 4.5 μM BA. Biomass increase in 14-day-culture was calculated and ursolic acid (UA) content was determined by HPLC and MS. The growth rate and UA accumulation was found to be significant in the two genotypes. The compact biomass of both genotypes demonstrated a much slower growth rate and a lower UA accumulation than the friable biomasses. The accumulation of UA in suspension culture was constant in time when derived from the friable callus but it declined, when derived from the compact callus. This revised version was published online in June 2006 with corrections to the Cover Date. |
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| Keywords: | callus plant cell suspension culture Salvia officinalis ursolic acid |
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