Assessment of oxidative DNA damage formation by organic complex mixtures from airborne particles PM10 |
| |
Authors: | Alena G belov , Zuzana Valovi
ov , Juraj L baj, Gabriela Ba
ov , Blanka Binkov ,Peter B. Farmer |
| |
Affiliation: | aLaboratory of Mutagenesis and Carcinogenesis, Cancer Research Institute of the Slovak Academy of Sciences, Bratislava, Slovakia;bLaboratory of Ecotoxicology, Institute of Experimental Medicine of the Academy of Sciences of the Czech Republic, Prague, Czech Republic;cCancer Biomarkers and Prevention Group, Biocentre, University of Leicester, UK |
| |
Abstract: | The free radical generating activity of airborne particulate matter (PM10) has been proposed as a primary mechanism in biological activity of ambient air pollution. In an effort to determine the impact of the complex mixtures of extractable organic matter (EOM) from airborne particles on oxidative damage to DNA, the level of 8-oxo-2′-deoxyguanosine (8-oxodG), the most prevalent and stable oxidative lesion, was measured in the human metabolically competent cell line Hep G2. Cultured cells were exposed to equivalent EOM concentrations (5–150 μg/ml) and oxidative DNA damage was analyzed using a modified single cell gel electrophoresis (SCGE), which involves the incubation of whole cell DNA with repair specific DNA endonuclease, which cleaves oxidized DNA at the sites of 8-oxodG. EOMs were extracted from PM10 collected daily (24 h intervals) in three European cities: Prague (Czech Republic, two monitoring sites, Libuš and Smíchov), Košice (Slovak Republic) and Sofia (Bulgaria) during 3-month sampling periods in the winter and summer seasons. No substantial time- and dose-dependent increase of oxidative DNA lesions was detected in EOM-treated cells with the exception of the EOM collected at the monitoring site Košice, summer sampling. In this case, 2 h cell exposure to EOM resulted in a slight but significant increase of oxidative DNA damage at three from total of six concentrations. The mean 8-oxodG values at these concentrations ranged from 15.3 to 26.1 per 106 nucleotides with a value 3.5 per 106 nucleotides in untreated cells. B[a]P, the positive control, induced a variable but insignificant increase of oxidative DNA damage in Hep G2 cell (approximately 1.6-fold increase over control value).Based on these data we believe that EOM samples extracted from airborne particle PM10 play probably only a marginal role in oxidative stress generation and oxidative lesion formation to DNA. However, adsorbed organic compounds can undergo various interactions (additive or synergistic) with other PM components or physical factors (UV-A radiation) and in this way they might enhance/multiply the adverse health effects of air pollution. |
| |
Keywords: | Ambient air particle (PM10) Oxidative DNA damage 8-Oxo-2′ -deoxyguanosine Single cell gel electrophoresis |
本文献已被 ScienceDirect 等数据库收录! |
|