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Effects and modes of action of citrus limonoids against Leptinotarsa decemlineata
Authors:YONG-BIAO LIU  A. RANDALL ALFORD  MOHAMMED S. RAJAB  MICHAEL D. BENTLEY
Affiliation:Department of Entomology;*Department of Chemistry, University of Maine
Abstract:ABSTRACT Limonin and its five derivatives were evaluated in feeding bioassays for depression effects on feeding, growth and development of the fourth stadium Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae). Epilimonol and limonin diosphenol were most effective in depression of feeding and growth, with ED50s 10 and 8 μg/cm2. At a dosage of 31.6 μg/cm2, epilimonol, limonin diosphenol and limonin caused both significant feeding reduction and developmental delay, while deoxylimonol, tetrahydrolimonin and limonol were not effective. Mouth-part coating and antifeedant bioassays show that feeding inhibition at host acceptance level seems to be the primary mode of action of epilimonol and limonin diosphenol.
Keywords:Antifeedant    limonoid    Leptinotarsa decemlineata    Colorado potato beetle
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