Immunohistochemical identification of neurons containing corticotropin-releasing factor in the rat hypothalamus |
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Authors: | Dr. M. Kawata K. Hashimoto J. Takahara Y. Sano |
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Affiliation: | 1. Department of Anatomy, Kyoto Prefectural University of Medicine, Kyoto, Japan 3. Third Department of Internal Medicine, Okayama University Medical School, Okayama, Japan
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Abstract: | A specific rabbit anti-CRF serum and the immunoperoxidase technique were used to show that CRF-containing neurons are mainly distributed in the paraventricular and supraoptic nuclei of the rat hypothalamus. In addition, immunoreactive neurons are scattered in other hypothalamic regions. These neurons are 20--30 micrometers in diameter. From the present and previous investigations it may be concluded that the hypothalamic magnocellular nuclei, i.e., paraventricular and supraoptic, and other hypothalamic accessory nuclei, are the producing sites not only for vasopressin and oxytocin, but also for corticotropin-releasing factor. |
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