Interaction of calmodulin with lactoferrin. |
| |
Authors: | A de Lillo J M Tejerina J F Fierro |
| |
Affiliation: | Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Spain. |
| |
Abstract: | Calmodulin, as a major intracellular calcium-binding protein, regulates many Ca(2+)-dependent enzymes and plays an important role in a wide spectrum of cellular functions of the eukaryotes. Interaction between calmodulin and human lactoferrin, a 78 kDa protein with antibacterial properties, was found in the presence of Ca2+ using (i) a method for the detection of calmodulin binding proteins with biotinylated calmodulin, (ii) affinity chromatography on an agarose-calmodulin column with subsequent detection by an enzyme-linked immunosorbent assay (ELISA). The binding of calmodulin to lactoferrin blocked the ability of lactoferrin to agglutinate Micrococcus lysodeikticus. |
| |
Keywords: | |
|
|