| 草坪草生物技术研究进展 |
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| 引用本文： | 钱海丰,薛庆中.草坪草生物技术研究进展[J].生物技术通报,2002(4):26-29,33. |
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| 作者姓名： | 钱海丰 薛庆中 |
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| 作者单位： | 浙江大学农学系,杭州,310029 |
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| 基金项目： | 国家自然科学基金 ( 39870 42 1)项目资助 |
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| 摘 要： | 草坪草转基因方法主要有原生质体融合法，基因枪法和农杆菌介导法。抗生素（潮霉素，G418)和除草剂（bialaphos,ppt)都可用于草坪草也可能存在生态风险性。
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| 关 键 词： | 草坪草 生物技术 研究进展 原生质体 转基因 |
Advancement of Turfgrass Biotechnology |
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| Qian Haifeng Xue Qingzhong.Advancement of Turfgrass Biotechnology[J].Biotechnology Bulletin,2002(4):26-29,33. |
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| Authors: | Qian Haifeng Xue Qingzhong |
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| Abstract: | Advances in genetic transformation of turfgrass, like other major crop species, have been made due to the development of plant regeneration from embryogenic tissue cultures. Three conventional methods of gene delivery, direct DNA uptake by protoplasts, biolistic transformation and Agrobacterium-mediated transformation have been used successfully to produce several species of transgenic turfgrass. In transformation, antibiotics (hygromycin or G418), and herbicides (bialaphos, or ppt) are used to select transformed cell lines. Gus gene and GFP gene are always used as reported genes. However, transgenic turfgrass may also have potential environmental risks. |
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| Keywords: | Turfgrass Protoplast Transformation |
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