活性氧对巨噬细胞呼吸爆发影响及云芝多糖的保护作用

用化学发光法观察到叔丁基氢过氧化物对培养的小鼠腹腔巨噬细胞呼吸爆发有强烈的抑制作用。云芝多糖经腹腔注射后,能增强巨噬细胞呼吸爆发功能对叔丁基氢过氧化物损伤的抵抗力。云芝多糖处理的巨噬细胞谷胱甘肽过氧化物酶基础活力显著提高,在叔丁基氢过氧化物作用下,云芝多糖处理的巨噬细胞仍有较高的谷胱甘肽过氧化物酶活力。说明巨噬细胞的免疫功能与谷胱甘肽过氧化物酶活力有关,非特异性免疫多糖可提高细胞抗氧化能力,减轻活性氧损伤作用。

INHIBITION OF REACTIVE OXYGEN SPECIES ON RESPIRATORY BURST OF MACROPHAGE AND PROTECTION OF POLYSACCHARIDE FROM CORIOLUS VERSICOLOR

Using a luminol-dependent chemiluminescence assay we found tert-butylhydroperoxide to be a strong inhibitor of respiratory burst of the mouse peritoneal macrophage. However, the protection against the inhibition of respiratory burst induced by tert-butylhydrope-roxide was enhanced after the intraperitoneal injection of polysaccharide from Coriolus versicolor. Further investigation exhibited that glutathione peroxidase activity was markly elevated in PSK-treated macrophages, Meanwhile, higher activity of glutathione peroxidase was maintained in PSK-treated macrophages incubated with tert-butylhydroperoxide. These results suggested that the immunological function of macrophage was related to the activity of glutathione peroxidase. Non-specific immunopolysaccharide might prevent macrophage from damage induced by reactive oxygen species by enhancing antioxidation capacity.