| 中国三种实验用小型猪线粒体DNAD-loop多态性分析 |
| |
| 引用本文： | 刘中禄,魏泓,曾养志,王爱德,甘世祥. 中国三种实验用小型猪线粒体DNAD-loop多态性分析[J]. 遗传, 2001, 23(2): 123-127 |
| |
| 作者姓名： | 刘中禄 魏泓 曾养志 王爱德 甘世祥 |
| |
| 作者单位： | 1.第三军医大学实验动物中心，重庆 400038;2.云南农业大学动物资源学院，昆明 650201;3.广西大学动物科学系,南宁 530005;4.贵阳中医学院实验动物中心，贵阳 5500021.Laboratory Animal Center,Third Military Medical University , Chongqing 400038, China;2.Yunna Agriculture University , Kunming 650201,China;3.Guangxi University,Nanning 530005 China;4.Guiyang College of Traditional Chinese Medicine,Guiyang 550002,China |
| |
| 基金项目： | The project supported by National “973” Key Project Program(G200016106) and National “9,5” Key Program (96-A23-06-01) and National Natural Science Foundation of China(NSFC)(No.39830190,39770398) |
| |
| 摘 要： | 分析中国三种实验用小型猪线粒体DNA（mtDNA）D-loop的多态性，建立各品种品系猪的遗传标记，为各品种、品系猪的鉴别提供依据。应用PCR技术分别对西双版纳近交系小耳猪、广西巴马小型猪、贵州小型香猪和长白猪的血液总DNA样品中mtDNAD-loop进行扩增，用23种限制性内切酶消化，观察其酶切多态。PCR扩增其mtDNAD-loop5′端227bp高变区域，应用PCR?SSCP和PCR直接测序分析，观察其单链构象多态和序列多态。结果显示：三种小型猪之间未见酶切长度多态、单链构象多态和序列多态。与长白猪之间表现出单链构象多态和序列多态。本研究认为：三种实验用小型猪之间mtDNA多态性贫乏，证明其亲源关系很近，在母系起源和进化上有一致性，应用PCR RFLP、PCR SSCP和PCR直接测序分析，尚不能作为三种实验用小型猪品种、品系鉴定的依据，但与长白猪等欧系猪比较有一定差异。Abstract：the present study is to analyze the polymorphism of the mtDNA D-loop in three breeds of laboratory miniature pigs in China , and to establish its cytoplasmic DNA markers to distinguish among them . The polymorphism of mtDNA D-loop and its 5′-end high variable regions were detected by PCR-RFLP , PCR-SSCP and PCR-direct sequencing on Xishuangbanna Small-ear inbred pig, Guizhou miniature Xiang pig , Guangxi Bama miniature pig and Landrace.There was no polymorphism obtained among or within three breeds of Chinese laboratory miniature pigs besides Landrace. It is concluded that the polymorphism of mtDNA D-loop within the three breeds of Chinese laboratory miniature pigs is poor , These methods cannot be used to distinguish among them , but it can be used to distinguish them from Landrace.
|
| 关 键 词： | 小型猪 线粒体DNA控制区 聚合酶链反应 Miniature pig 多态性 Keywords |
| 文章编号： | 0253-9772(2001)02-0123-05 |
| 修稿时间： | 2000-04-21 |
Analysis of the Polymorphism of mtDNA D-loop in Three Breeds of Laboratory Miniature Pig in China |
| |
| LIU Zhong-lu,WEI Hong,ZENG Yang-zi,WANG Ai-de,GAN Shi-xiang. Analysis of the Polymorphism of mtDNA D-loop in Three Breeds of Laboratory Miniature Pig in China[J]. Hereditas, 2001, 23(2): 123-127 |
| |
| Authors: | LIU Zhong-lu WEI Hong ZENG Yang-zi WANG Ai-de GAN Shi-xiang |
| |
| Affiliation: | LIU Zhong-lu1 ,WEI Hong1,ZENG Yang-zi2,WANG Ai-de3,GAN Shi-xiang4 |
| |
| Abstract: | the present study is to analyze the polymorphism of the mtDNA D-loop in three breeds of laboratory miniature pigs in China , and to establish its cytoplasmic DNA markers to distinguish among them . The polymorphism of mtDNA D-loop and its 5′-end high variable regions were detected by PCR-RFLP , PCR-SSCP and PCR-direct sequencing on Xishuangbanna Small-ear inbred pig, Guizhou miniature Xiang pig , Guangxi Bama miniature pig and Landrace.There was no polymorphism obtained among or within three breeds of Chinese laboratory miniature pigs besides Landrace. It is concluded that the polymorphism of mtDNA D-loop within the three breeds of Chinese laboratory miniature pigs is poor , These methods cannot be used to distinguish among them , but it can be used to distinguish them from Landrace. |
| |
| Keywords: | Miniature pig PCR mtDNA D-loop polymorphism |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《遗传》浏览原始摘要信息 |
|
点击此处可从《遗传》下载全文 |
|
