Relation between the impairment of creatine kinase activity and lipid peroxidation in the membranes of heart mitochondria and sarcoplasmic reticulum in experimental ischemia

Experiments were conducted on rabbits under conditions of acute restriction of coronary blood flow (intravenous injection of vasopressin in a dose of 0.5 U/kg) and preliminary administration (1 h before vasopressin) of PP-256-Na antioxidant preparation. The activity of creatine kinase isoenzymes bound with sarcoplasmic reticulum and mitochondria membranes were studied as well as the content of diene conjugates in these membranes and accumulation of malonic dialdehyde. It is shown that an essential decrease in the enzyme activity in the membranes occurs against a background of a considerable increase in the content of diene conjugates and accumulation of malonic dialdehyde. A preliminary administration of the antioxidant produces a pronounced protective effect on the enzyme activity.