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Measurements of sediment toxicity of autotrophic and heterotrophic picoplankton by epifluorescence microscopy |
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| Authors: | Severn S R T Munawar M Mayfield C I |
| Institution: | (1) CB Research International Corporation, P. O. Box 2010, V8L 3S3 Sidney, B. C., Canada;(2) Great Lakes Laboratory for Fisheries & Aquatic Sciences, Fisheries & Oceans Canada, Canada Centre for Inland Waters, L7R 4A6 Burlington, Ontario, Canada;(3) Biology Department, University of Waterloo, N2L 3G1 Waterloo, Ontario, Canada |
| Abstract: | Sediment toxicity from Toronto (Ontario) and Toledo (Ohio) harbours to autotrophic and heterotrophic picoplankton was evaluated simultaneously using epifluorescent microscopy. The approach is a simple, fast and effective combination of autofluorescence and fluorescence probes - 1-anilino-8-naphthalene sulfonic acid. The procedure is ideally suited for use with sediment slurries since it can differentiate fluorescent biotic material against a background of abiotic sediment particles and detritus. |
| Keywords: | sediment toxicity autotrophic and heterotrophic picoplankton epifluorescence microscopy |
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