Biosynthesis of mono- and sesquiterpenes in carrot roots and leaves (Daucus carota L.): metabolic cross talk of cytosolic mevalonate and plastidial methylerythritol phosphate pathways |
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Authors: | Hampel Daniela Mosandl Armin Wüst Matthias |
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Institution: | Institut für Lebensmittelchemie, Johann Wolfgang Goethe-Universit?t, Marie-Curie-Strasse 9, D-60439 Frankfurt/Main, Germany. |
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Abstract: | The biosynthesis of the monoterpenes terpinolene and myrcene and the sesquiterpene beta-caryophyllene in roots and leaves of two carrot varieties (Daucus carota L. cultivars Bolero and Kazan) were investigated by in vivo feeding experiments with 5,5-2H2]-mevalonic acid lactone (d2-MVL) and 5,5-2H2]-1-deoxy-D-xylulose (d2-DOX). The volatiles of the tissues were extracted by stir bar sorptive extraction and analyzed using thermal desorption-multidimensional gas chromatography-mass spectrometry. The experiments demonstrate independent de novo-biosynthesis of terpenoids in carrot roots and in carrot leaves. In both plant tissues monoterpenes are biosynthesized exclusively via the 1-deoxy-D-xylulose/2-C-methyl-D-erythritol-4-phosphate (DOXP/MEP) pathway, whereas sesquiterpenes are generated by the classical mevalonic acid pathway as well as by the DOXP/MEP route. A more detailed investigation of carrot root tissues revealed that the biosynthesis of terpenes is mainly localized in the phloem. Nevertheless, in xylem a de novo-biosynthesis of terpenes was detectable as well, even in the absence of oil ducts in this tissue. |
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Keywords: | Daucus carota L Umbelliferae d-xylulose" target="_blank">1-Deoxy-d-xylulose Mevalonic acid Stir bar sorptive extraction Thermal desorption-multidimensional gas chromatography-mass spectrometry |
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